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Frequent posttranslational modifications include methylation of the ε-amino group, giving methyl- , dimethyl- , and trimethyllysine The latter occurs in calmodulin Different posttranslational modifications at lysine residues embody acetylation , sumoylation , and ubiquitination Collagen incorporates hydroxylysine , which is derived from lysine by lysyl hydroxylase - Glycosylation of hydroxylysine residues in the endoplasmic reticulum or Golgi apparatus is used to mark sure proteins for secretion from the cell In opsins like rhodopsin and the visual opsins (encoded by the genes OPN1SW , OPN1MW , and OPN1LW ), retinaldehyde kinds a Schiff base with a conserved lysine residue, and interaction of light with the retinylidene group causes sign transduction in color vision (See visible cycle for details).
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Ensure all of the purification buffers are at four °C and perform the protein purification within the chilly room to avoid protein denaturation. BE AWARE: This avoids the coupling of amino acids within the further cycles with the membrane as an alternative of the growing peptide chain. NOTICE: After the coupling of the first C-terminal amino acid of all peptides to the amino group of the membrane, the amino teams between the spots and in addition some of the amino groups within the spot areas don't type bonds with the amino acids.
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